Free services Ligation Calculator

Free services Ligation Calculator

The Ligation Calculator: What Is It?

 Molecular biologists and other laboratory experts can use the Ligation Calculator to determine the ideal quantities of insert and vector DNA needed for successful DNA ligation operations. This calculator allows users to enter many parameters, including DNA concentrations, lengths, and desired molar ratios. It then calculates the precise amounts required for each component, making ligation experiments more accurate and efficient.

Ligation Calculator

Insert DNA concentration (ng/µL) i Enter the concentration of your insert DNA in nanograms per microliter (ng/µL).

Vector DNA concentration (ng/µL) i Enter the concentration of your vector DNA in nanograms per microliter (ng/µL).

Insert length (bp) i Enter the length of your insert DNA in base pairs (bp).

Vector length (bp) i Enter the length of your vector DNA in base pairs (bp).

Desired insert:vector molar ratio i Select the desired molar ratio of insert DNA to vector DNA. 1:1 3:1 5:1

Total reaction volume (µL) i Enter the total reaction volume in microliters (µL).

Results:

Volume of insert to add: µL

Volume of vector to add: µL

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