Free services Ligation Calculator

 

Free services Ligation Calculator

Ligation Calculator

Insert DNA concentration (ng/µL) i Enter the concentration of your insert DNA in nanograms per microliter (ng/µL).

Vector DNA concentration (ng/µL) i Enter the concentration of your vector DNA in nanograms per microliter (ng/µL).

Insert length (bp) i Enter the length of your insert DNA in base pairs (bp).

Vector length (bp) i Enter the length of your vector DNA in base pairs (bp).

Desired insert:vector molar ratio i Select the desired molar ratio of insert DNA to vector DNA. 1:1 3:1 5:1

Total reaction volume (µL) i Enter the total reaction volume in microliters (µL).

Results:

Volume of insert to add: µL

Volume of vector to add: µL

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