Free services Annealing Temperature Calculator

 

Free services Annealing Temperature Calculator

The Annealing Temperature Calculator: What Is It?

 The ideal temperature for the annealing phase of a polymerase chain reaction (PCR) can be found with the use of the Annealing Temperature Calculator. Researchers and lab staff working in the fields of molecular biology and biotechnology use this calculator.

Annealing Temperature Calculator

Primer Sequence (Forward): ? Enter the nucleotide sequence for the forward primer.
Primer Sequence (Reverse): ? Enter the nucleotide sequence for the reverse primer.
Salt Concentration (M): ? Typically between 0.01-0.1 M.
Primer Concentration (µM): ? Typically between 0.1-1 µM.

Annealing Temperature (Ta): N/A °C

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